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Authentic mammalian proteins for high-end applications

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Production of biotherapeutics and their reagents using mammalian-based expression systems remains the mainstay of the industry to maintain post-translational modifications required for specific and efficacious drug targets.

Daresbury Proteins Ltd specializes in mammalian recombinant protein production and cell line development which can be transferred into a third party’s cGMP setting.

As an emerging biotech, we developed our proprietary approach while delivering various proteins for cutting-edge research. Our experience and know-how allow us to obtain difficult-to-express proteins in a short period of time with uncompromising quality. We have seen that our stable expression vector system can increase protein expression by up to 50-fold when compared to alternative mammalian expression systems. 

To our academic and industrial partners, we bring our extensive knowledge relating to expression of a wide range of product classes including those that are difficult to express, development of research cell lines and production of candidate targets.

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Novel Expression Platform For Mammalian Recombinants.

Our novel proprietary expression vectors support stable, high level expression of a broad range of product classes from key, industry focussed mammalian-based production systems.

Focussing on product quality, our proprietary expression technologies support high titre production using a rapid pool approach to deliver our clients targets without the need for additional clonal selection and expansion. Our aim is to support our clients goal of rapidly assessing the functionality of their candidates whilst reducing cost and risk by removing the barriers associated with clonal cell line development.

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Fact. Effective. Challenging Targets.

Daresbury Proteins can generate rapid pools and research cell lines from a range of production backgrounds including CHO-K1 and HEK293 and are happy to discuss each of our clients’ specific needs focussed on the production of high quality targets and reagents.

Our capabilities include:

  1. Sub-cloning of a coding DNA sequence into our proprietary vectors. We strongly recommend host-cell based codon optimisation of the coding DNA insert to increase expression level. This service is provided by our partner, Dapcel Inc, who use their award-winning codon-optimisation algorithm. Alternatively, we can use customer-provided DNA sequence and primers for PCR amplification to introduce into our expression vectors.

  2. Generation of stable rapid pools and cryopreservation of Master Cell Stock. If preferred by our clients, then we are able to support the generation of clonal cell lines for research purposes.

  3. Production of target recombinants and supporting analytical package to confirm suitability of candidate targets.

Please contact us with your specific project.



Active Proteins. Reliable Reagents.

We have produced excellent proteins which have been used in academic research and featured in high impact publications. Almost all of our catalogue proteins were one-of-a-kind when we produced them for university-based scientists, unavailable commercially elsewhere. As a result, our recombinants have been used as rare antigens to generate highly specific antibodies, develop unique assays and produce ground-breaking data.

Our proteins are supplied in a liquid buffered formulation. Secondary structures of our full-length soluble recombinants confer high stability even after days of storage at ambient temperature ensuring their authentic function and activity.

  1. Human recombinant Fibroblast Growth Factor Receptor 1, FGFR1b, (Uniprot P11362-19), aa 22-376, C-term 10xHis

  2. Human recombinant Fibroblast Growth Factor Receptor 1, FGFR1c, (Uniprot P11362-7), aa 22-374, C-term 10xHis

  3. Human recombinant Fibroblast Growth Factor Receptor 2, FGFR2b, (Uniprot P21802-3), aa 22-378, C-term 10xHis

  4. Human recombinant Fibroblast Growth Factor Receptor 2, FGFR2c, (Uniprot P21802-1), aa 22-377, C-term 10xHis

  5. Human recombinant Fibroblast Growth Factor Receptor 3, FGFR3b, (Uniprot P22607-2), aa 23-377, C-term 10xHis

  6. Human recombinant Fibroblast Growth Factor Receptor 3, FGFR3c, (Uniprot P22607-1), aa 23-375, C-term 10xHis

  7. Human recombinant Fibroblast Growth Factor Receptor 4, FGFR4, (Uniprot P22455), aa 22-369, C-term 10xHis

  8. Human recombinant Phospholipase A2 Receptor 1, PLA2R, (Uniprot Q13018), aa 21-1397, C-term 10xHis

  9. Human recombinant Phospholipase A2 Receptor 1, PLA2R, (Uniprot Q13018), aa 21-663, C-term 10xHis

  10. Human recombinant Phospholipase A2 Receptor 1, PLA2R, (Uniprot Q13018), aa 21-515, C-term 10xHis

  11. Human recombinant Phospholipase A2 Receptor 1, PLA2R, (Uniprot Q13018), aa 21-173, C-term 10xHis

  12. Human recombinant Thrombospondin Type-1 Domain-Containing Protein 7A, THSD7A, (Uniprot Q9UPZ6), aa 48-1606, C-term 10xHis

  13. Human recombinant Thrombospondin Type-1 Domain-Containing Protein 7A, THSD7A, (Uniprot Q9UPZ6), aa 48-1171, C-term 10xHis

  14. Human recombinant Vascular Adhesion Protein-1, VAP-1, (Uniprot Q16853), aa 29-763, N-term 10xHis

  15. Human recombinant Ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), CD73, (Uniprot P21589), aa 27-549, C-term 6xHis

  16. Human recombinant Amyloid Precursor Protein, alpha form, APPα, (Isoform APP695 Uniprot P05067-4), aa 18-612, N-term 10xHis

  17. Human recombinant Amyloid Precursor Protein, beta form, APPβ, (Isoform APP695 Uniprot P05067-4), aa 18-596, N-term 10xHis

  18. Human recombinant Autotaxin, Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2, ATX, ENPP2, (Uniprot Q13822), aa 49-863, C-term 10xHis 

  19. SARS-CoV-2 trimeric Spike protein, D614G mutant, European variant, S protein, S, (Uniprot P0DTC2), aa 16-1213, C-term 8xHis

  20. Human recombinant Angiotensin-converting Enzyme 2, ACE2 (Uniprot Q9BYF1), aa 19-740, C-term 10xHis

  21. SARS-CoV-2 trimeric Spike protein, Beta Variant of Concern, South Africa mutant, B.1.351 cluster, S protein, S, (Uniprot P0DTC2), aa 16-1211, C-term 8xHis.

  22. SARS-CoV-2 trimeric Spike protein, Gamma Variant of Concern, Brazil mutant, P.1 cluster, S protein, S, (Uniprot P0DTC2), aa 16-1211, C-term 8xHis.

  23. SARS-CoV-2 trimeric Spike protein, Lineage B.1.617, India cluster, S protein, S, (Uniprot P0DTC2), aa 16-1211, C-term 8xHis.

  24. SARS-CoV-2 trimeric Spike protein, Delta Variant of Concern, India mutant, B.1.617.2 cluster, S protein, S, (Uniprot P0DTC2), aa 16-1211, C-term 8xHis.

  25. SARS-CoV-2 trimeric Spike protein, Alpha Variant of Concern, UK mutant, B.1.1.7 cluster, S protein, S, (Uniprot P0DTC2), aa 16-1211, C-term 8xHis.

  26. SARS-CoV-2 trimeric Spike protein, Omicron Variant of Concern, B.1.1.529 cluster, 21K clade, S protein, S, (Uniprot P0DTC2), aa 16-1211, C-term 8xHis.



 Daniel J. Williamson, PhD 

Principal Scientist at Iksuda Therapeutics

 Iksuda Therapeutics partnered with Daresbury Proteins to create a stable cell line and generate a recombinant IgG1 human antibody. The expression system delivered substantially more material than was required by the project, which was completed on-time, on-budget and importantly, of an appropriate quality. Communication with Daresbury Proteins was excellent throughout the course of the project with detailed updates being provided on a regular basis whilst the team were able to accommodate changes in the work plan to suit the needs of the project. 

Dr Andrea Balogh

Semmelweis University, Budapest

The recombinant human ATX custom designed and manufactured by Daresbury protein in the shortest time possible, is reliable and stable, high quality product providing excellent results in repeated measurements and the best value for money.



Daresbury Proteins Ltd., Sci-Tech Daresbury, Daresbury Labs, Keckwick Lane, Warrington WA4 4AD, United Kingdom

+44 (0) 7398623734

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